ʻEhā ʻano o nā mea hoʻomaʻi hanu nucleic acid
Inoa mea kūʻai
HWTS-RT075-Hoʻomaloʻo-hoʻohuʻu 4 ʻano o nā mea hoʻomaʻi hanu nucleic acid Detection Kit (Fluorescence PCR)
Epidemiology
ʻO ka maʻi maʻi ʻo Corona Virus 2019, i kapa ʻia ʻo "COVID-19", e pili ana i ka maʻi pneumonia i hoʻokumu ʻia e ka maʻi SARS-CoV-2.ʻO SARS-CoV-2 kahi coronavirus pili i ka genus β.ʻO COVID-19 kahi maʻi maʻi ʻeha hanu, a maʻalahi ka heluna kanaka.I kēia manawa, ʻo ke kumu o ka maʻi ka nui o nā maʻi i loaʻa i ka maʻi e SARS-CoV-2, a ʻo ka poʻe maʻi asymptomatic paha ke kumu o ka maʻi.Ma muli o ka noiʻi epidemiological o kēia manawa, ʻo ka manawa incubation he 1-14 mau lā, ʻo ka hapa nui he 3-7 mau lā.ʻO ke kuni, ka ʻū maloʻo a me ka luhi nā hōʻike nui.Ua loaʻa i kekahi mau maʻi nā hōʻailona e like me ka nasal congestion, runny ihu, ʻeha ʻāʻī, myalgia a me ka diarrhea, etc.
ʻO ka maʻi influenza, i ʻike nui ʻia ʻo "flu", he maʻi ʻeha ʻeha i hoʻokumu ʻia e ka maʻi maʻi influenza.He ma'i lele loa.Hoʻouna nui ʻia ia e ka ʻu ʻana a me ke kihe ʻana.He mea maʻamau i ka pūnāwai a me ka hoʻoilo.Hoʻokaʻawale ʻia nā maʻi maʻi maʻi influenza A (IFV A), influenza B (IFV B), a me ka Influenza C (IFV C) ʻekolu ʻano, no nā maʻi maʻi paʻa a pau, ke kumu o ka maʻi kanaka no ka maʻi influenza A a me B, he hoʻokahi. - stranded, ʻāpana RNA virus.ʻO ka maʻi Influenza A he maʻi hanu nui, e like me H1N1, H3N2 a me nā ʻano subtype ʻē aʻe, hiki ke hoʻololi a maʻi i ka honua holoʻokoʻa.ʻO ka "Shift" e pili ana i ka hoʻololi ʻana o ka maʻi maʻi influenza A, ka hopena i ka puka ʻana mai o kahi maʻi hou "subtype".Ua māhele ʻia nā maʻi maʻi influenza B i ʻelua laina, ʻo Yamagata a me Victoria.ʻO ka maʻi Influenza B wale nō ka antigenic drift, a pale aku i ka nānā ʻana a me ka hoʻopau ʻana o ka ʻōnaehana pale kanaka ma o kona hoʻololi ʻana.Eia naʻe, ʻoi aku ka lohi o ka hoʻololi ʻana o ka maʻi maʻi influenza B ma mua o ka maʻi influenza A kanaka.Hiki i ka ma'i Influenza B ke ho'oulu i ka ma'i hanu kanaka a alaka'i i ka ma'i ahulau.
ʻO ka maʻi maʻi hanu hanu (RSV) he maʻi RNA, no ka ʻohana paramyxoviridae.Hoʻouna ʻia ia e nā kulu lewa a me ka pilina pili a ʻo ia ka pathogen nui o ka maʻi ʻeha haʻahaʻa o ka ʻōpū o nā pēpē.Hiki i nā kamaliʻi i loaʻa i ka RSV ke hoʻomohala i ka bronchiolitis koʻikoʻi a me ka maʻi pneumonia, pili i ka hānō i nā keiki.Loaʻa i nā pēpē nā hōʻailona koʻikoʻi, e like me ke kuni kiʻekiʻe, rhinitis, pharyngitis a me ka laryngitis, a laila bronchiolitis a me ka maʻi pneumonia.Hiki ke paʻakikī kekahi mau keiki maʻi me ka otitis media, pleurisy a me ka myocarditis, a me nā mea ʻē aʻe.
Kanal
FAM | SARS-CoV-2 |
VIC(HEX) | RSV |
CY5 | IFV A |
ROX | IFV B |
NED | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
Waihona | 2-8°C |
Kau-ola | 12 mahina |
ʻAno Hoʻohālike | ʻOropharyngeal swab |
Ct | ≤38 |
LoD | SARS-CoV-2: 150 Kope/mL ʻO ka maʻi maʻi Influenza A/Maʻi maʻi influenza B/Maʻi hoʻomaʻamaʻa hanu: 300 Kopi/mL |
kikoo | ʻAʻohe ʻano hoʻololi keʻa me ka coronavirus kanaka SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, metapneumovirus kanaka, enterovirus A, B, C, D, ma'i pulmonary kanaka, ma'i epstein-barr, ma'i ma'i ma'i, ma'i ma'i cytomegalo kanaka, rotavirus, norovirus, ma'i parotitis, ma'i varicella-zoster, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, s.pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci a me cryptococcus hānau hou a me ke kanaka genomic nucleic acid. |
Nā mea hana pili | Hoʻohana ʻia ʻo Biosystems 7500 Pūnaehana PCR manawa maoli, Pūnaehana PCR i hoʻohana ʻia 7500 wikiwiki loa, ʻo QuantStudio®5 Pūnaehana PCR manawa maoli. |
Holo Hana
Koho 1.
Manaʻo ʻia ka hoʻoneʻe ʻana: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Koho 2.
Manaʻo ʻia ka mea hoʻoneʻe ʻoki: Nucleic Acid Extraction a i ʻole Purification Reagent (YDP302) na Tiangen Biotech(Beijing) Co., Ltd.