HCV Genotyping
inoa mea kūʻai
HWTS-HP004-HCV Puke ʻImi ʻImi (Fluorescence PCR)
Epidemiology
No ka ʻohana flaviviridae ka maʻi Hepatitis C (HCV), a ʻo kona genome he kaula RNA maikaʻi hoʻokahi, hiki ke hoʻololi ʻia.Aia ka ma'i i loko o nā hepatocytes, nā leukocytes serum a me ka plasma o ka po'e ma'i.Hiki ke hoʻololi ʻia nā genes HCV a hiki ke hoʻokaʻawale ʻia i 6 mau genotypes a me nā ʻano subtypes.Hoʻohana nā genotypes HCV like ʻole i nā ʻano hoʻoponopono a me nā papa o ka lāʻau DAA.No laila, ma mua o ka mālama ʻia ʻana o nā maʻi me ka DAA antiviral therapy, pono e ʻike ʻia ka genotype HCV, a no nā poʻe maʻi me ke ʻano 1, pono e hoʻokaʻawale inā he ʻano 1a a i ʻole type 1b.
Kanal
| FAM | ʻAno 1b, ʻano 2a |
| ROX | ʻAno 6a, ʻano 3a |
| VIC/HEX | Mana Kūloko, ʻAno 3b |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ I ka pōʻeleʻele |
| Kau-ola | 9 mahina |
| ʻAno Hoʻohālike | Serum, Plasma |
| Ct | ≤36 |
| CV | ≤5.0％ |
| LoD | 200 IU/mL |
| kikoo | E hoʻohana i kēia pahu no ka ʻike ʻana i nā maʻi maʻi ʻē aʻe a i ʻole nā laʻana bacteria e like me: human cytomegalovirus, Epstein-Barr virus, human immunodeficiency virus, hepatitis B virus, hepatitis A virus, syphilis, human herpes virus type 6, herpes simplex virus type 1, simplex Herpes virus ʻano 2, maʻi influenza A, Propionibacterium acnes, Staphylococcus aureus, Candida albicans, etc. ʻAʻole maikaʻi nā hopena. |
| Nā mea hana pili | Hiki iā ia ke hoʻohālikelike i nā mea hana PCR fluorescent mainstream ma ka mākeke. ABI 7500 Pūnaehana PCR manawa maoli ʻO ABI 7500 ʻO nā Pūnaehana PCR ʻOiaʻiʻo wikiwiki SLAN-96P Pūnaehana PCR Manawa maoli QuantStudio®5 Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻIke PCR manawa maoli MA-6000 ʻOiaʻiʻo ka nui o ka wela wela BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
E kākau i kāu leka ma aneʻi a hoʻouna mai iā mākou
