ʻO ke kanaka BCR-ABL Fusion Gene Mutation
Inoa mea kūʻai
HWTS-GE010A-Human BCR-ABL Fusion Gene Mutation Detection Kit (Fluorescence PCR )
Epidemiology
ʻO ka myelogenousleukemia maʻamau (CML) he maʻi maʻi clonal ʻino o nā cell stem hematopoietic.ʻOi aku ma mua o 95% o nā mea maʻi CML e lawe i ka chromosome Philadelphia (Ph) i loko o kā lākou mau koko.ʻO ka pathogenesis nui o CML penei: Hoʻokumu ʻia ka BCR-ABL fusion gene ma ka unuhi ʻana ma waena o ka abl proto-oncogene (Abelson murine leukemia viral oncogene homolog 1) ma ka lima lōʻihi o ka chromosome 9 (9q34) a me ka ʻāpana puʻupuʻu breakpoint ( BCR) gene ma ka lima lōʻihi o ka chromosome 22 (22q11);ʻO ka protein fusion i hoʻopaʻa ʻia e kēia moʻo he tyrosine kinase (TK), a hoʻāla i kāna mau ala hōʻailona o lalo (e like me RAS, PI3K, a me JAK/STAT) e hāpai i ka mahele cell a keʻakeʻa i ka apoptosis cell, e hoʻonui nui ai nā cell, a laila e hoʻoulu ai. ka hiki ʻana mai o CML .ʻO BCR-ABL kekahi o nā hōʻailona diagnostical koʻikoʻi o CML.ʻO ka hoʻololi ikaika o kona pae transcript he hōʻailona hilinaʻi no ka hoʻoholo wānana o ka maʻi leukemia a hiki ke hoʻohana ʻia e wānana i ka hoʻi hou ʻana o ka maʻi leukemia ma hope o ka mālama ʻana.
Kanal
| FAM | BCR-ABL fusion gene |
| VIC/HEX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | Ka wai: ≤-18 ℃ I ka pōʻeleʻele |
| Kau-ola | Ka wai: 9 mahina |
| ʻAno Hoʻohālike | Nā laʻana o ka iwi iwi |
| LoD | 1000 Kope/ mL |
| kikoo
| ʻAʻohe mea hoʻopā kea me nā genes hui ʻē aʻe TEL-AML1, E2A-PBX1, MLL-AF4, AML1-ETO, a me PML-RARa |
| Nā mea hana pili | Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio® 5 Pūnaehana PCR Manawa maoli SLAN-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli MA-6000 ʻOiaʻiʻo ka nui o ka wela wela BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
