ʻO ke kanaka EML4-ALK Fusion Gene Mutation
Inoa mea kūʻai
HWTS-TM006-Human EML4-ALK Fusion Gene Mutation Detection Kit(Fluorescence PCR)
Palapala
CE
Epidemiology
Hoʻohana ʻia kēia kime e ʻike pono i nā ʻano mutation 12 o EML4-ALK fusion gene i loko o nā laʻana o nā maʻi maʻi maʻi maʻi ʻaʻai liʻiliʻi liʻiliʻi i loko o ka vitro.ʻO nā hopena hōʻike no ka hōʻike lapaʻau wale nō a ʻaʻole pono e hoʻohana ʻia ma ke kumu hoʻokahi no ka mālama ʻana i nā maʻi.Pono nā kauka lapaʻau e hoʻoholo i nā hopena hōʻike ma muli o nā kumu e like me ke kūlana o ka mea maʻi, nā hōʻailona lāʻau, ka pane ʻana i ka lāʻau, a me nā hōʻailona hōʻike hoʻokolohua ʻē aʻe.'O ka ma'i 'a'ai 'o ia ka ma'i ma'i ma'amau ma'amau ma ka honua holo'oko'a, a he 80% ~ 85% o nā ma'i 'a'ole li'ili'i li'ili'i ka ma'i 'a'ai ma'i ma'i ma'i pūpū (NSCLC).ʻO ka hui pū ʻana o ka echinoderm microtubule-associated protein-like 4 (EML4) a me anaplastic lymphoma kinase (ALK) kahi pahuhopu hou i NSCLC, EML4 a me ALK aia i loko o ke kanaka ka P21 a me ka P23 band ma ka chromosome 2 a ua hoʻokaʻawale ʻia ma kahi o 12.7 miliona mau papa kumu.Ua ʻike ʻia he 20 mau ʻano like ʻole, a ma waena o ia mau mea maʻamau ka 12 fusion mutants ma ka Papa 1, kahi mutant 1 (E13; A20) ka mea maʻamau, a ukali ʻia e nā mutants 3a a me 3b (E6; A20), e helu ana ma kahi o 33% a me 29% o nā mea maʻi me EML4-ALK fusion gene NSCLC, kēlā me kēia.ʻO nā mea hoʻopaneʻe ALK i hōʻike ʻia e Crizotinib he mau lāʻau liʻiliʻi i hoʻolālā ʻia no ka hoʻololi ʻana o ka gene fusion ALK.Ma ke kaohi ʻana i ka hana o ka ALK tyrosine kinase māhele, ke ālai ʻana i kona mau ala hōʻailona maʻamau i lalo, a laila ke kāohi nei i ka ulu ʻana o nā pūnaewele tumo, e hoʻokō i ka lāʻau lapaʻau no nā ʻōpū.Ua hōʻike ʻia nā haʻawina hoʻomaʻamaʻa ʻo Crizotinib i ʻoi aku ma mua o 61% i nā poʻe maʻi me nā mutation fusion EML4-ALK, ʻoiai ʻaʻohe hopena i nā mea maʻi ʻano hihiu.No laila, ʻo ka ʻike ʻana i ka mutation fusion EML4-ALK ke kumu a me ke kumu no ke alakaʻi ʻana i ka hoʻohana ʻana i nā lāʻau lapaʻau Crizotinib.
Kanal
| FAM | Paʻa pānaʻi 1, 2 |
| VIC(HEX) | Paʻa pānaʻi 2 |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 9 mahina |
| ʻAno Hoʻohālike | ʻiʻo pathological i hoʻokomo ʻia i ka paraffin a i ʻole nā ʻāpana ʻāpana |
| CV | <5.0% |
| Ct | ≤38 |
| LoD | Hiki i kēia pahu ke ʻike i nā hoʻololi fusion ma lalo o 20 kope. |
| Nā mea hana pili: | Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems SLAN ®-96P Pūnaehana PCR Manawa maoli QuantStudio™ 5 Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli MA-6000 ʻOiaʻiʻo ka nui o ka wela wela BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Manaʻo ʻia ka mea hoʻoneʻe hoʻoneʻe: RNeasy FFPE Kit (73504) na QIAGEN, Paraffin-embedded Tissue Sections Total RNA Extraction Kit(DP439) by Tiangen Biotech(Beijing) Co.,Ltd.
