ʻO ka Middle East Respiratory Syndrome Coronavirus Nucleic Acid

ʻO ka wehewehe pōkole:

Hoʻohana ʻia ka pahu no ka ʻike qualitative o MERS coronavirus nucleic acid i loko o nā swabs nasopharyngeal me ka Middle East Respiratory Syndrome (MERS) coronavirus.


Huahana Huahana

Huahana Huahana

Inoa mea kūʻai

HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit(Fluorescent PCR)

Epidemiology

ʻO ka Middle East respiratory syndrome coronavirus (MERS-CoV), he β-coronavirus e kumuʻO ka maʻi hanu i loko o nā kānaka, ua ʻike mua ʻia i kahi maʻi kāne Saudi Arabian 60 makahiki i make ma 24th July, 2012. ʻO ka hōʻike hōʻike ʻana o ka maʻi MERS-CoV mai ka asymptomatic state a i ʻole nā ​​​​hōʻailona hanu māmā a hiki i ka maʻi ʻeha nui a hiki i ka make.

Kanal

FAM MERS virus RNA
VIC(HEX)

Hoomalu Kuloko

Nā ʻāpana ʻenehana

Waihona

≤-18 ℃ I ka pōʻeleʻele

Kau-ola

9 mahina

ʻAno Hoʻohālike

ʻOhi hou ʻia nā swabs nasopharyngeal

CV

≤5.0%

Ct

≤38

LoD

1000 kope/mL

kikoo

ʻAʻohe mea hoʻohālikelike me nā coronaviruses kanaka coronavirus SARSr-CoV a me nā pathogens maʻamau.

Nā mea hana pili:

Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli

Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Pūnaehana PCR Manawa maoli

SLAN-96P Pūnaehana PCR Manawa maoli

LightCycler®480 Pūnaehana PCR manawa maoli

LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli

MA-6000 ʻOiaʻiʻo ka nui o ka wela wela

BioRad CFX96 Pūnaehana PCR Manawa maoli

BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli

Holo Hana

Koho 1.

Manaʻo ʻia ka mea hoʻoulu hou: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction a i ʻole Purification Reagent (YDP315-R) na Tiangen Biotech (Beijing) Co., Ltd.

Koho 2.

Manaʻo ʻia ka hoʻoulu ʻana: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).


  • Mua:
  • Aʻe:

  • E kākau i kāu leka ma aneʻi a hoʻouna mai iā mākou