ʻO ka Middle East Respiratory Syndrome Coronavirus Nucleic Acid

ʻO ka wehewehe pōkole:

Hoʻohana ʻia ka pahu no ka ʻike qualitative o MERS coronavirus nucleic acid i loko o nā swabs nasopharyngeal me ka Middle East Respiratory Syndrome (MERS) coronavirus.

E hoʻouna i ka leka uila iā mākou

Huahana Huahana

Inoa mea kūʻai

HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit(Fluorescent PCR)

Epidemiology

ʻO ka Middle East respiratory syndrome coronavirus (MERS-CoV), he β-coronavirus e kumuʻO ka maʻi hanu i loko o nā kānaka, ua ʻike mua ʻia i kahi maʻi kāne Saudi Arabian 60 makahiki i make ma 24th July, 2012. ʻO ka hōʻike hōʻike ʻana o ka maʻi MERS-CoV mai ka asymptomatic state a i ʻole nā hōʻailona hanu māmā a hiki i ka maʻi ʻeha nui a hiki i ka make.

Kanal

FAM	MERS virus RNA
VIC(HEX)	Hoomalu Kuloko

Nā ʻāpana ʻenehana

Waihona	≤-18 ℃ I ka pōʻeleʻele
Kau-ola	9 mahina
ʻAno Hoʻohālike	ʻOhi hou ʻia nā swabs nasopharyngeal
CV	≤5.0%
Ct	≤38
LoD	1000 kope/mL
kikoo	ʻAʻohe mea hoʻohālikelike me nā coronaviruses kanaka coronavirus SARSr-CoV a me nā pathogens maʻamau.
Nā mea hana pili:	Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR Manawa maoli SLAN-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli MA-6000 ʻOiaʻiʻo ka nui o ka wela wela BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli

Holo Hana

Koho 1.

Manaʻo ʻia ka mea hoʻoulu hou: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction a i ʻole Purification Reagent (YDP315-R) na Tiangen Biotech (Beijing) Co., Ltd.

Koho 2.

Manaʻo ʻia ka hoʻoulu ʻana: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).

Mua: Nā ʻano like ʻole SARS-CoV-2

Aʻe: SARS-CoV-2 IgM/IgG Antibody

E kākau i kāu leka ma aneʻi a hoʻouna mai iā mākou