ʻO ka pale ʻana o ka Mycobacterium Tuberculosis INH

ʻO ka wehewehe pōkole:

Hoʻohana ʻia kēia kit no ka ʻike pono ʻana i ka hoʻololi ʻana o ka ʻakika amino 315 o ka gene katG (K315G>C) a me ka hoʻololi ʻana o ka ʻāpana o ka mea hoʻolaha o ka gene InhA (- 15 C>T).


Huahana Huahana

Huahana Huahana

inoa mea kūʻai

HWTS-RT002A-Mycobacterium Tuberculosis Isoniazid Resistance Detection Kit (Fluorescence PCR)

Epidemiology

ʻO Isoniazid, kahi lāʻau anti-tuberculosis kī i hoʻokomo ʻia i ka makahiki 1952, ʻo ia kekahi o nā lāʻau lapaʻau maikaʻi loa no ka mālama ʻana i ka maʻi maʻi maʻi a me kahi lāʻau hoʻokahi no ka maʻi maʻi latent.

ʻO KatG ka mea nui e hoʻopili ana i ka catalase-peroxidase a me ka mutation gene katG hiki ke hoʻoikaika i ka synthesis o ka mycolic acid cell wall, e hana i ka maʻi bacteria i ka isoniazid.Hoʻopili maikaʻi ʻia ka ʻōlelo KatG me nā loli i ka INH-MIC, a ʻo ka hoʻemi ʻana o 2-fold i ka hōʻike katG e hopena i ka hoʻonui iki ʻana i 2-fold ma MIC.ʻO kekahi kumu o ka pale ʻana i ka isoniazid i ka mycobacterium tuberculosis i ka wā i hoʻokomo ʻia ai ke kumu, ka holoi ʻana a i ʻole ka hoʻololi ʻana i loko o ka InhA gene locus o mycobacterium tuberculosis.

Kanal

ROX inhA (-15C>T) kahua·
CY5

katG (315G>C).

VIC (HEX)

IS6110

Nā ʻāpana ʻenehana

Waihona ≤-18 ℃ I ka pōʻeleʻele
Kau-ola

12 mahina

ʻAno Hoʻohālike

Sputum

CV ≤5.0%
LoD

1 × 103bacteria/mL

kikoo No-cross reactivity me ka hoʻololi ʻana o nā kahua kūʻē lāʻau ʻehā (511, 516, 526 a me 531) o ka gene rpoB ma waho o ka pae ʻike o ka pahu ʻike.

Nā mea hana pili:

Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli

Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Pūnaehana PCR Manawa maoli

SLAN-96P Pūnaehana PCR Manawa maoli

LightCycler®480 Pūnaehana PCR manawa maoli

LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli

MA-6000 ʻOiaʻiʻo ka nui o ka wela wela

BioRad CFX96 Pūnaehana PCR Manawa maoli

BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli

Holo Hana

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