ʻO ka maʻi maʻi maʻi SARS-CoV-2 A influenza B Hui Pū ʻIa Nucleic
Inoa mea kūʻai
HWTS-RT060A-SARS-CoV-2 influenza A influenza B Nucleic Acid Combined Detection Kit (Fluorescence PCR)
Epidemiology
ʻO ka maʻi maʻi ʻo Corona Virus 2019 (COVID-19) ke kumu e SARS-CoV-2 nona ka β Coronavirus o ka genus.ʻO COVID-19 kahi maʻi maʻi maʻi hanu, a maʻalahi ka lehulehu.I kēia manawa, ʻo nā maʻi maʻi maʻi SARS-CoV-2 ke kumu nui o ka maʻi, a ʻo nā mea maʻi asymptomatic hiki ke lilo i kumu o ka maʻi.Ma muli o ka noiʻi epidemiological o kēia manawa, ʻo ka manawa incubation he 1-14 mau lā, ʻo ka hapa nui he 3-7 mau lā.ʻO nā hiʻohiʻona nui o ke kuni, ka ʻū maloʻo a me ka luhi.Loaʻa i kekahi mau maʻi nā hōʻailona e like me ka nasal congestion, runny ihu, ʻeha ʻāʻī, myalgia a me ka diarrhea.
ʻO ka maʻi influenza kahi maʻi hanu ʻeha i hoʻokumu ʻia e ka maʻi influenza.He ma'i ma'i nui loa ia a laha nui ma ke ku'u a me ke kihe.He mea maʻamau i ka pūnāwai a me ka hoʻoilo.ʻEkolu mau ʻano o ka Influenza, ʻo ka maʻi maʻi A (IFV A), ka maʻi maʻi B (IFV B) a me ka maʻi maʻi C (IFV C), no ka ʻohana ortomyxovirus lāua ʻelua.ʻO ka maʻi maʻi A a me B, ʻo ia nā maʻi maʻi RNA i hoʻokahi-stranded, nā kumu nui o nā maʻi kanaka.ʻO ka maʻi influenza A he maʻi maʻi ʻeha hanu, me H1N1, H3N2 a me nā ʻano ʻano ʻē aʻe, maʻalahi ke hoʻololi.ʻO ka maʻi maʻi honua, ʻo "shift" e pili ana i ka mutation o ka influenza A, ka hopena i kahi "subtype" viral hou.Ua māhele ʻia ʻo Influenza B i ʻelua mau laina: Yamagata a me Victoria.ʻO ka maʻi maʻi B he antigenic drift wale nō, a pale lākou i ka nānā ʻana a me ka hoʻopau ʻia e ka ʻōnaehana pale kanaka ma o ka mutation.Akā ʻoi aku ka lohi o nā maʻi maʻi influenza B ma mua o ka maʻi maʻi A i ke kanaka, ʻo ia hoʻi ke kumu o ka maʻi hanu a me nā maʻi maʻi i loko o ke kanaka.
Kanal
FAM | SARS-CoV-2 |
ROX | IFV B |
CY5 | IFV A |
VIC(HEX) | Nā genes Mana Kuloko |
Nā ʻāpana ʻenehana
Waihona | Ka wai: ≤-18 ℃ I ka pōʻeleʻele |
Lyophilization: ≤30 ℃ i ka pōʻeleʻele | |
Kau-ola | Ka wai: 9 mahina |
Lyophilization: 12 mahina | |
ʻAno Hoʻohālike | Nasopharyngeal swabs, Oropharyngeal swabs |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 300 kope/mL |
kikoo | Ua hōʻike ʻia nā hopena hōʻike keʻa ua kūpono ka pahu me ka coronavirus kanaka SARSr- CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, respiratory syncytial virus A a me B, parainfluenza virus 1, 2 a 3, rhinovirusA, B a me C, adenovirus 1, 2, 3, 4, 5, 7 a me 55, metapneumovirus kanaka, enterovirus A, B, C a me D, virus cytoplasmic pulmonary kanaka, virus EB, measles virus Human cytomegalovirus, rotavirus, norovirus, mumps virus, varicella zoster virus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus al. fumigabratus reaction. ma waena o Pneumocystis yersini a me Cryptococcus neoformans. |
Nā mea hana pili: | Hiki iā ia ke hoʻohālikelike i nā mea hana PCR fluorescent mainstream ma ka mākeke. Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR Manawa maoli SLAN-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli MA-6000 ʻOiaʻiʻo ka nui o ka wela wela BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Koho 1.
Manaʻo ʻia ka hoʻoneʻe ʻana: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Koho 2.
Manaʻo ʻia ka mea hoʻoneʻe ʻoki: Nucleic Acid Extraction a i ʻole Purification Reagent (YDP302) na Tiangen Biotech(Beijing) Co., Ltd.