Nā ʻano like ʻole SARS-CoV-2
Inoa mea kūʻai
HWTS-RT072A-SARS-CoV-2 pahu ʻike ʻokoʻa (Fluorescence PCR)
Palapala
CE
Epidemiology
Ua hoʻolaha ʻia ka novel coronavirus (SARS-CoV-2) ma kahi ākea a puni ka honua.I ke kaʻina o ka hoʻolaha ʻana, kū mau nā mutation hou, e hopena i nā ʻano hou.Hoʻohana nui ʻia kēia huahana no ka ʻike kōkua a me ka hoʻokaʻawale ʻana i nā hihia e pili ana i ka maʻi ma hope o ka hoʻolaha nui ʻana o Alpha, Beta, Gamma, Delta a me Omicron mutant strains mai Dekemaba 2020.
Kanal
FAM | N501Y, HV69-70del |
CY5 | 211-212del, K417N |
VIC(HEX) | E484K, Mana Kūloko |
ROX | P681H, L452R |
Nā ʻāpana ʻenehana
Waihona | ≤-18 ℃ I ka pōʻeleʻele |
Kau-ola | 9 mahina |
ʻAno Hoʻohālike | nasopharyngeal swabs, oropharyngeal swabs |
CV | ≤5.0% |
Ct | ≤38 |
LoD | 1000 kope/mL |
kikoo | ʻAʻohe mea hoʻohālikelike me nā coronaviruses kanaka SARS-CoV a me nā pathogens maʻamau. |
Nā mea hana pili: | QuantStudio™5 Pūnaehana PCR Manawa maoli SLAN ®-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli MA-6000 ʻOiaʻiʻo ka nui o ka wela wela BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Koho 1.
Manaʻo ʻia ka hoʻoneʻe ʻana: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Koho 2.
Manaʻo ʻia ka mea hoʻoneʻe ʻoki: Nucleic Acid Extraction a i ʻole Purification Reagent (YDP302) na Tiangen Biotech(Beijing) Co., Ltd.