Staphylococcus Aureus a me ka Methicillin-Resistant Staphylococcus Aureus Nucleic Acid
Inoa mea kūʻai
HWTS-OT062-Staphylococcus Aureus a me Methicillin-Resistant Staphylococcus Aureus Nucleic Acid Detection Kit (Fluorescence PCR)
Palapala
CE
Epidemiology
ʻO Staphylococcus aureus kekahi o nā maʻi pathogenic koʻikoʻi o ka maʻi nosocomial.No ka staphylococcus 'o Staphylococcus aureus (SA) a he 'elele ia no ka ma'i bacteria Gram-positive, hiki ke ho'opuka i nā 'ano mea 'awa'awa a me nā enzyme invasive.Loaʻa i ka bacteria nā hiʻohiʻona o ka hoʻolaha ākea, ka pathogenicity ikaika a me ka nui o ke kū'ē kiʻekiʻe.ʻO ke ʻano nuclease Thermotable (nuc) kahi gene i mālama nui ʻia o staphylococcus aureus.I nā makahiki i hala iho nei, ma muli o ka hoʻohana nui ʻana o nā hormones a me ka hoʻomākaukau ʻana i ka immune a me ka hōʻino ʻana i nā lāʻau antibiotic ākea ākea, ua piʻi aʻe nā maʻi nosocomial i hoʻokumu ʻia e Methicillin-resistant Staphylococcus aureus (MRSA) i Staphylococcus.ʻO ka awelika o ka ʻike maka o MRSA he 30.2% ma 2019 ma Kina.Ua māhele ʻia ʻo MRSA i MRSA pili i ke olakino (HA-MRSA), MRSA pili kaiaulu (CA-MRSA), a me MRSA pili i nā holoholona (LA-MRSA).ʻO CA-MRSA, HA-MRSA, LA-MRSA nā ʻokoʻa nui i ka microbiology, kū'ē bacteria (e laʻa, hōʻike ʻo HA-MRSA i ke kūʻē ʻana i nā lāʻau he nui ma mua o CA-MRSA) a me nā hiʻohiʻona lapaʻau (e laʻa me ka pae o ka maʻi).Wahi a kēia mau hiʻohiʻona, hiki ke hoʻokaʻawale ʻia ʻo CA-MRSA a me HA-MRSA.Eia naʻe, ke emi nei ka ʻokoʻa ma waena o CA-MRSA a me HA-MRSA ma muli o ka neʻe mau ʻana o nā kānaka ma waena o nā haukapila a me nā kaiāulu.ʻO ka MRSA ke kū'ē i nā lāʻau he nui,ʻaʻole wale i nā lāʻau'alopelo β-lactam, akā i nā aminoglycosides, macrolides, tetracyclines a me nā quinolones i nāʻano likeʻole.Nui nā ʻokoʻa kūloko i nā helu kūʻē lāʻau a me nā ʻano ʻokoʻa.
ʻO ka methicillin resistance mecA gene ka mea koʻikoʻi i ka pale ʻana i ka staphylococcal.Lawe ʻia ke ʻano ma luna o kahi mea hoʻokalakupua kelepona kūʻokoʻa (SCCmec), ka mea e hoʻopili ai i ka penicillin-binding protein 2a (PBP2a) a he haʻahaʻa haʻahaʻa ia i nā antibiotics β-lactam, no laila ʻaʻole hiki i nā lāʻau antimicrobial ke kāohi i ka synthesis o ka pā peptidoglycan layer. ka hopena i ka lāʻau kūʻē.
Kanal
| FAM | gene mecA pale methicillin |
| CY5 | staphylococcus aureus nuc gene |
| VIC/HEX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | Ka wai: ≤-18 ℃ |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | sputum, ʻili a me ka ʻiʻo palupalu nā laʻana maʻi, a me nā laʻana koko holoʻokoʻa |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL |
| kikoo | ʻAʻohe mea e hana hou ai me nā poʻe'ē aʻe'ē aʻe e like me nā mea'ē aʻe e like me ka huiʻana o ka staphylococus ʻO Rabilis, komo i ka cloacacter calacae, straptoccus pneumononiae , enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Nā mea hana pili | Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli QuantStudio®5 Pūnaehana PCR Manawa maoli SLAN-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli MA-6000 ʻOiaʻiʻo ka nui o ka wela wela BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
