ʻO Enterococcus kū'ē iā Vancomycin a me Gene kū'ē i ka lāʻau
Inoa mea kūʻai
HWTS-OT090-Vancomycin-resistant Enterococcus and Drug-resistant Gene Detection Kit (Fluorescence PCR)
Epidemiology
ʻIke ʻia ʻo Resistance to Drug ma ke ʻano he lāʻau kūʻē, pili i ke kūʻē ʻana o ka bacteria i ka hana o nā lāʻau antibacterial.Ke hiki mai ke kū'ē i ka lāʻau, e hoʻemi nui ʻia ka hopena chemotherapy o nā lāʻau lapaʻau.Hoʻokaʻawale ʻia ke kū'ē i ka lāʻau i loko o ke kū'ē kū'ē a me ke kū'ē i loaʻa.Hoʻoholo ʻia ke kūpaʻa intrinsic e nā genes chromosomal bacteria, i hele mai kēlā hanauna i kēia hanauna, a ʻaʻole e loli.ʻO ke kū'ē i loaʻa ma muli o ka ʻoiaʻiʻo ma hope o ka hoʻopili ʻana me nā lāʻau lapaʻau, hoʻololi ka bacteria i kā lākou mau ala metabolic i ʻole e pepehi ʻia lākou e nā lāʻau antibiotic.
Loaʻa i nā genes kū'ē vancomycin VanA a me VanB ke kū'ē i ka lāʻau lapaʻau, ma waena o VanA e hōʻike ana i nā kūlana kiʻekiʻe o ke kū'ē i vancomycin a me teicoplanin, hōʻikeʻo VanB i nā kūlana likeʻole o ke kū'ē i ka vancomycin, a ua maʻalahi i ka teicoplanin.Hoʻohana pinepine ʻia ʻo Vancomycin no ka mālama ʻana i nā maʻi bacterial Gram-positive, akā ma muli o ka puka ʻana mai o ka vancomycin-resistant enterococci (VRE), ʻoi aku ka enterococcus faecalis a me enterococcus faecium, ʻoi aku ka nui ma mua o 90%, ua lawe mai ia i nā pilikia nui hou i ka lāʻau lapaʻau. .I kēia manawa, ʻaʻohe lāʻau antibacterial kūikawā no ka mālama ʻana iā VRE.He aha hou aʻe, hiki i ka VRE ke hoʻouna i nā genes kūʻē i ka lāʻau i nā enterococci ʻē aʻe a i ʻole nā bacteria Gram-positive ʻē aʻe.
Kanal
| FAM | ʻO Enterococci kū'ē i ka vancomycin (VRE): Enterococcus faecalis a me Enterococcus faecium |
| VIC/HEX | Hoomalu Kuloko |
| CY5 | vancomycin resistance gene VanB |
| ROX | vancomycin resistance gene VanA |
Nā ʻāpana ʻenehana
| Waihona | Ka wai: ≤-18 ℃ |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | sputum, koko, mimi a i ʻole nā ʻāpana maʻemaʻe |
| CV | ≤5.0% |
| Ct | ≤36 |
| LoD | 103CFU/mL |
| kikoo | ʻAʻohe hana keʻa me nā maʻi maʻi hanu ʻē aʻe e like me klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, streptococcus pneumoniae, neisseria meningitidis, staphylococcus aureus, klebsiella oxytoca, haemophilus influenzae, A. junii, A. haemophilia p. pseudomonas fluorescens, candida albicans, chlamydia pneumoniae, respiratory adenovirus, a i ʻole nā laʻana i loaʻa nā genes kūʻē lāʻau CTX, mecA, SME, Nā Laʻana o SHV a me TEM. |
| Nā mea hana pili | Hoʻohana ʻia ʻo Biosystems 7500 Pūnaehana PCR manawa maoli SLAN-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli |
Holo Hana
Manaʻo ʻia nā reagents unuhi: Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) .
