Hoʻololi kanaka BRAF Gene V600E
Inoa mea kūʻai
HWTS-TM007-Human BRAF Gene V600E Mutation Detection Kit(Fluorescence PCR)
Palapala
CE
Epidemiology
Ua ʻoi aku ma mua o 30 mau ʻano hoʻololi BRAF, kahi o 90% aia ma exon 15, kahi i manaʻo ʻia ai ka hoʻololi ʻana o V600E ʻo ia ka mutation maʻamau, ʻo ia hoʻi, ʻo thymine (T) ma ke kūlana 1799 ma exon 15 i hoʻololi ʻia i adenine (A), ka hopena o ka hoʻololi ʻana o valine (V) ma ke kūlana 600 e ka glutamic acid (E) i loko o ka huahana protein.Loaʻa pinepine ʻia nā hoʻololi BRAF i nā maʻi maʻi ʻino e like me ka melanoma, ka maʻi maʻi colorectal, ka maʻi maʻi thyroid, a me ka maʻi ʻaʻai māmā.ʻO ka hoʻomaopopo ʻana i ka hoʻololi ʻana o ka gene BRAF ua lilo i mea pono e nānā i nā EGFR-TKI a me BRAF gene mutation-targeted lāʻau i loko o ka lāʻau lapaʻau i hoʻopaʻa ʻia i ka lāʻau lapaʻau no nā poʻe maʻi i hiki ke pōmaikaʻi.
Kanal
| FAM | Hoʻololi V600E, hoʻomalu i loko |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 9 mahina |
| ʻAno Hoʻohālike | nā laʻana o ka ʻiʻo pathological i hoʻokomo ʻia i ka paraffin |
| CV | <5.0% |
| Ct | ≤38 |
| LoD | E hoʻohana i nā kits e ʻike i ka mana maikaʻi o LoD.a) ma lalo o 3ng/μL wild-type background, 1% mutation rate hiki ke ʻike ʻia i ka paʻa paʻa;b) ma lalo o 1% mutation rate, ka mutation o 1×103Kope/mL ma ke ano hihiu o 1×105Hiki ke ʻike paʻa ʻia nā kope/mL i loko o ka pale pane;c) hiki i ka IC Reaction Buffer ke ʻike i ka palena haʻahaʻa haʻahaʻa haʻahaʻa haʻahaʻa haʻahaʻa haʻahaʻa ka mana maikaʻi SW3 o ka mana kūloko o ka hui. |
| Nā mea hana pili: | Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoliHoʻohana ʻia ʻo Biosystems 7300 PCR manawa maoli Pūnaehana, QuantStudio® 5 Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli BioRad CFX96 Pūnaehana PCR Manawa maoli |
Holo Hana
ʻO nā mea hoʻihoʻi hou ʻia: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) i hana ʻia e Tiangen Biotech(Beijing) Co., Ltd.
