Hoʻololi kanaka BRAF Gene V600E

ʻO ka wehewehe pōkole:

Hoʻohana ʻia kēia pahu hoʻāʻo e ʻike pono i ka hoʻololi ʻana o ka BRAF gene V600E i loko o nā ʻano kikoo paraffin-embedded o ka melanoma kanaka, colorectal cancer, thyroid cancer and lung cancer in vitro.


Huahana Huahana

Huahana Huahana

Inoa mea kūʻai

HWTS-TM007-Human BRAF Gene V600E Mutation Detection Kit(Fluorescence PCR)

Palapala

CE

Epidemiology

Ua ʻoi aku ma mua o 30 mau ʻano hoʻololi BRAF, kahi o 90% aia ma exon 15, kahi i manaʻo ʻia ai ka hoʻololi ʻana o V600E ʻo ia ka mutation maʻamau, ʻo ia hoʻi, ʻo thymine (T) ma ke kūlana 1799 ma exon 15 i hoʻololi ʻia i adenine (A), ka hopena o ka hoʻololi ʻana o valine (V) ma ke kūlana 600 e ka glutamic acid (E) i loko o ka huahana protein.Loaʻa pinepine ʻia nā hoʻololi BRAF i nā maʻi maʻi ʻino e like me ka melanoma, ka maʻi maʻi colorectal, ka maʻi maʻi thyroid, a me ka maʻi ʻaʻai māmā.ʻO ka hoʻomaopopo ʻana i ka hoʻololi ʻana o ka gene BRAF ua lilo i mea pono e nānā i nā EGFR-TKI a me BRAF gene mutation-targeted lāʻau i loko o ka lāʻau lapaʻau i hoʻopaʻa ʻia i ka lāʻau lapaʻau no nā poʻe maʻi i hiki ke pōmaikaʻi.

Kanal

FAM Hoʻololi V600E, hoʻomalu i loko

Nā ʻāpana ʻenehana

Waihona

≤-18 ℃

Kau-ola

9 mahina

ʻAno Hoʻohālike

nā laʻana o ka ʻiʻo pathological i hoʻokomo ʻia i ka paraffin

CV

<5.0%

Ct

≤38

LoD

E hoʻohana i nā kits e ʻike i ka mana maikaʻi o LoD.a) ma lalo o 3ng/μL wild-type background, 1% mutation rate hiki ke ʻike ʻia i ka paʻa paʻa;b) ma lalo o 1% mutation rate, ka mutation o 1×103Kope/mL ma ke ano hihiu o 1×105Hiki ke ʻike paʻa ʻia nā kope/mL i loko o ka pale pane;c) hiki i ka IC Reaction Buffer ke ʻike i ka palena haʻahaʻa haʻahaʻa haʻahaʻa haʻahaʻa haʻahaʻa haʻahaʻa ka mana maikaʻi SW3 o ka mana kūloko o ka hui.

Nā mea hana pili:

Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoliHoʻohana ʻia ʻo Biosystems 7300 PCR manawa maoli

Pūnaehana, QuantStudio® 5 Pūnaehana PCR Manawa maoli

LightCycler®480 Pūnaehana PCR manawa maoli

BioRad CFX96 Pūnaehana PCR Manawa maoli

Holo Hana

ʻO nā mea hoʻihoʻi hou ʻia: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) i hana ʻia e Tiangen Biotech(Beijing) Co., Ltd.


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