Kanaka Leukocyte Antigen B27 Nucleic Acid

ʻO ka wehewehe pōkole:

Hoʻohana ʻia kēia pahu no ka ʻike qualitative o ka DNA i nā subtypes antigen leukocyte kanaka HLA-B*2702, HLA-B*2704 a me HLA-B*2705.


Huahana Huahana

Huahana Huahana

Inoa mea kūʻai

HWTS-GE011A-Human Leukocyte Antigen B27 Nucleic Acid Detection Kit (Fluorescence PCR)

Epidemiology

ʻO Ankylosing spondylitis (AS) kahi maʻi ʻehaʻeha holomua e hoʻopiʻi nui ana i ka spine a hiki ke komo i nā hui sacroiliac a me nā hono a puni i nā pae like ʻole.Ua hōʻike ʻia e hōʻike ana ʻo AS i ka hōʻuluʻulu ʻohana maopopo a pili loa i ka antigen leukocyte kanaka HLA-B27.I loko o ke kanaka, ʻoi aku ma mua o 70 mau ʻano ʻano ʻano HLA-B27 i ʻike ʻia a ʻike ʻia, a o lākou, HLA-B*2702, HLA-B*2704 a me HLA-B*2705 nā ʻano subtypes maʻamau e pili ana i ka maʻi.Ma Kina, Singapore, Iapana a me Taiwan apana o Kina, ʻo ka subtype maʻamau o HLA-B27 ʻo HLA-B*2704, helu ʻia ma kahi o 54%, a ukali ʻia e HLA-B*2705, ʻo ia ka helu ma kahi o 41%.Hiki i kēia pahu ke ʻike i ka DNA ma nā ʻano subtypes HLA-B*2702, HLA-B*2704 a me HLA-B*2705, akā ʻaʻole ia e hoʻokaʻawale iā lākou mai kekahi i kekahi.

Kanal

FAM HLA-B27
VIC/HEX Hoomalu Kuloko

Nā ʻāpana ʻenehana

Waihona Ka wai: ≤-18 ℃ I ka pōʻeleʻele
Kau-ola Ka wai: 18 mahina
ʻAno Hoʻohālike nā laʻana koko holoʻokoʻa
Ct ≤40
CV ≤5.0%
LoD 1ng/μL

kikoo

 

ʻAʻole e hoʻopilikia ʻia nā hopena hoʻāʻo ma kēia pahu e ka hemoglobin (<800g/L), bilirubin (<700μmol/L), a me nā lipids koko/triglycerides (<7mmol/L) i ke koko.
Nā mea hana pili Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli

Hoʻohana ʻia ʻo Biosystem StepOne Pūnaehana PCR manawa maoli

LightCycler®480 Pūnaehana PCR manawa maoli

Pūnaehana Q-PCR Agilent-Stratagene Mx3000P


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