Kanaka Methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene

ʻO ka wehewehe pōkole:

Hoʻolālā ʻia ka pahu no ka ʻike in vitro qualitative o nā genes methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 i loko o nā pūnaewele exfoliated intestinal i loko o nā mea hoʻohālike o ke kanaka.


Huahana Huahana

Huahana Huahana

Inoa mea kūʻai

HWTS-OT077-Human Methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene Detection Kit (Fluorescence PCR)

Palapala

CE

Epidemiology

I ka poe makua, oi aku mamua o 10 8 intestinal epithelial cell e haule mai ka intestinal paia i kela la i keia la, a ua excreted me ka feces ma o ka nui bowel peristalsis.Ma muli o ka hāʻule ʻana o ka ʻōpū o ka ʻōpū o ka hoʻonui ʻia ʻana, ʻo ka ʻōpala o nā mea maʻi maʻi ʻōpū he nui nā maʻi maʻi a me nā ʻāpana cell abnormal, ʻo ia ke kumu kumu no ka ʻike ʻana i ka ʻōpala paʻa.Ua ʻike ʻia nā haʻawina ʻo ka hoʻololi ʻana i ka methylation o nā mea hoʻolaha gene he hanana mua ia i ka tumorigenesis, a ʻo nā mea genetic i loaʻa mai nā kumu hoʻohālike o nā maʻi maʻi maʻi colorectal hiki ke hōʻike i ke ʻano o ka maʻi kanesa i loko o ka ʻōpū ma mua.

ʻO NDRG4, i kapa ʻia ʻo SMAP-8 a me BDM1, ʻo ia kekahi o nā lālā ʻehā o ka ʻohana gene NDRG (NDRG1-4), i hōʻike ʻia e pili ana i ka hoʻonui ʻana o ka cell, ka ʻokoʻa, ka ulu ʻana a me ke kaumaha.Ua hōʻoia ʻia ʻo ka NDRG4 methylation kahi mea biomarker no ka ʻike ʻole ʻana i ka maʻi maʻi colorectal ma nā ʻāpana stool.

He lālā ʻo SEPT9 o ka ʻohana gene Septin, nona ka liʻiliʻi he 13 mau genes e hoʻopili ana i kahi kikowaena GTPase i mālama ʻia e hiki ke hoʻopaʻa i nā protein pili cytoskeleton, a pili pū me ka māhele cell a me ka tumorigenesis.Ua ʻike ʻia nā haʻawina ua hoʻonui nui ʻia ka maʻi methylated Septin9 gene i loko o nā laʻana stool mai nā maʻi me ka maʻi maʻi colorectal.

ʻO nā protein pili i pili i ka frizzled huna (sFRPs) he mau polokina hiki ke hoʻoheheʻe ʻia he papa o nā antagonists ala Wnt ma muli o kā lākou homology kiʻekiʻe i ka mea hoʻokipa frizzled (Fz) no ka hōʻailona Wnt.ʻO ka inactivation o ka SFRP gene ka hopena i ka hoʻōla ʻole ʻia o ka hōʻailona Wnt pili me ka maʻi maʻi colorectal.I kēia manawa, hiki ke hoʻohana ʻia ka SFRP2 methylation i loko o ka stool ma ke ʻano he biomarker non-invasive no ka ʻike ʻana i ka maʻi maʻi colorectal.

He lālā ʻo BMP3 o ka ʻohana super TGF-B a no laila he kuleana koʻikoʻi i ka ulu ʻana o ka embryonic ma o ka hoʻoulu ʻana a me ka hoʻohua ʻana i ka iwi mua.ʻO ka BMP3 ka hypermethylated i ka maʻi maʻi colorectal a hiki ke hoʻohana ʻia ma ke ʻano he hōʻailona koʻikoʻi.

ʻO SDC2 kahi proteoglycan heparan sulfate ma luna o ke kelepona i komo i ka hoʻoponopono ʻana i nā kaʻina hana physiological a me nā pathological.ʻO ke kino i hana ʻia me ka hoʻonui ʻana o ka cell, ka ʻokoʻa, ka adhesion, ka hui cytoskeletal, ka neʻe ʻana, ka hoʻōla ʻana i ka ʻeha, ke kamaʻilio cell-matrix, angiogenesis;ʻO nā kaʻina hana pathological e pili ana i ka mumū a me ke kanesa.ʻOi aku ka kiʻekiʻe o ka pae methylation o ka gene SDC2 i loko o nā ʻiʻo maʻi maʻi colorectal ma mua o nā mea maʻamau.

Kanal

Hoʻopaʻa pānaʻi A

VIC/HEX methylated NDRG4 gene
ROX methylated SEPT9 gene
CY5 hoʻomalu kūloko

Paʻa pānaʻi B

VIC/HEX methylated SFRP2 gene
ROX methylated BMP3 gene
FAM methylated SDC2 gene
CY5 hoʻomalu kūloko

Ka wehewehe

Gene

Kaila hōʻailona

Ct Waiwai

Ka wehewehe

NDRG4

VIC (HEX)

Ct waiwai≤38

NDRG4 maikaʻi

Waiwai Ct>38 a i ʻole ke kala

NDRG4 maikaʻi ʻole

SEPAT9

ROX

Ct waiwai≤38

SEPT9 maikaʻi

Waiwai Ct>38 a i ʻole ke kala

SEPT9 maikaʻi ʻole

SFRP2

VIC (HEX)

Ct waiwai≤38

SFRP2 maikaʻi

Waiwai Ct>38 a i ʻole ke kala

SFRP2 ʻino

Nā ʻāpana ʻenehana

Waihona Ka wai: ≤-18 ℃
Kau-ola 9 mahina
ʻAno Hoʻohālike Laʻana Stool
CV ≤5.0%
kikoo ʻAʻohe ʻano hoʻololi ʻana me ka maʻi maʻi ate, ka maʻi maʻi bile duct, ka maʻi maʻi thyroid a me ka maʻi ʻāʻī
Nā mea hana pili QuantStudio ®5 Pūnaehana PCR Manawa maoli

SLAN-96P Pūnaehana PCR Manawa maoli

LightCycler®480 Pūnaehana PCR manawa maoli

LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli

MA-6000 ʻOiaʻiʻo ka nui o ka wela wela

Holo Hana

Manaʻo ʻia ka hoʻoulu ʻana: Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS- 3006).


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