ʻO ke kanaka ROS1 Fusion Gene Mutation

ʻO ka wehewehe pōkole:

Hoʻohana ʻia kēia kit i ka ʻike in vitro qualitative o 14 ʻano o ka ROS1 fusion gene mutations i loko o nā laʻana maʻi maʻi ʻaʻai liʻiliʻi liʻiliʻi liʻiliʻi (Papa 1).ʻO nā hopena hōʻike no ka hōʻike lapaʻau wale nō a ʻaʻole pono e hoʻohana ʻia ma ke kumu hoʻokahi no ka mālama ʻana i nā maʻi.


Huahana Huahana

Huahana Huahana

Inoa mea kūʻai

HWTS-TM009-Human ROS1 Fusion Gene Mutation Detection Kit (Fluorescence PCR)

Palapala

CE

Epidemiology

ʻO ROS1 kahi transmembrane tyrosine kinase o ka ʻohana insulin receptor.Ua hōʻoia ʻia ʻo ROS1 fusion gene ma ke ʻano koʻikoʻi ʻē aʻe ʻaʻole liʻiliʻi liʻiliʻi liʻiliʻi ka maʻi maʻi maʻi maʻi maʻi maʻi maʻi maʻi.Ma ke ʻano he ʻelele o kahi subtype molecular hou, ʻo ka hanana o ROS1 fusion gene ma NSCLC Ma kahi o 1% a 2% ROS1 ka nui o ka hoʻoponopono hou ʻana i kāna mau exons 32, 34, 35 a me 36. Ma hope o ka hoʻohui ʻia ʻana me nā genes e like me CD74, EZR, SLC34A2, a me SDC4, e hoʻomau ia i ka hoʻoulu ʻana i ka ʻāpana ROS1 tyrosine kinase.Hiki i ka ROS1 kinase ho'ā 'ole ke ho'āla i nā ala hōʻailona o lalo e like me RAS/MAPK/ERK, PI3K/Akt/mTOR, a me JAK3/STAT3, no laila ke komo ʻana i ka hoʻomāhuahua ʻana, ka ʻokoʻa a me ka metastasis o nā pūnaewele tumo, a me ka hoʻoulu ʻana i ka maʻi kanesa.Ma waena o ROS1 fusion mutations, CD74-ROS1 helu ma kahi o 42%, EZR ma kahi o 15%, SLC34A2 ma kahi o 12%, a me SDC4 ma kahi o 7%.Ua hōʻike ʻia nā haʻawina ʻo ka ATP-binding site o ka catalytic domain o ROS1 kinase a me ka ATP-binding site o ALK kinase he homology a hiki i ka 77%, no laila, ʻo ka ALK tyrosine kinase liʻiliʻi mole inhibitor crizotinib a pēlā aku i maopopo ka hopena curative. i ka mālama ʻana i ka NSCLC me ka fusion mutation o ROS1.No laila, ʻo ka ʻike ʻana i nā mutation fusion ROS1 ke kumu a me ke kumu no ke alakaʻi ʻana i ka hoʻohana ʻana i nā lāʻau lapaʻau crizotinib.

Kanal

FAM Paʻa Paʻa 1, 2, 3 a me 4
VIC(HEX) Paʻa Paʻa 4

Nā ʻāpana ʻenehana

Waihona

≤-18 ℃

Kau-ola

9 mahina

ʻAno Hoʻohālike

ʻiʻo pathological i hoʻokomo ʻia i ka paraffin a i ʻole nā ​​ʻāpana ʻāpana

CV

<5.0%

Ct

≤38

LoD

Hiki i kēia pahu ke ʻike i nā hoʻololi fusion ma lalo o 20 kope.

Nā mea hana pili:

Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoliHoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems

SLAN ®-96P Pūnaehana PCR Manawa maoli

QuantStudio™ 5 Pūnaehana PCR Manawa maoli

LightCycler®480 Pūnaehana PCR manawa maoli

LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli

MA-6000 ʻOiaʻiʻo ka nui o ka wela wela

BioRad CFX96 Pūnaehana PCR Manawa maoli

BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli

Holo Hana

Manaʻo ʻia ka mea hoʻihoʻi hou: RNeasy FFPE Kit (73504) mai QIAGEN, Paraffin Embedded Tissue Section Total RNA Extraction Kit(DP439) mai Tiangen Biotech(Beijing) Co.,Ltd.


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