ʻO ka pale ʻana o ka Mycobacterium Tuberculosis INH
inoa mea kūʻai
HWTS-RT002A-Mycobacterium Tuberculosis Isoniazid Resistance Detection Kit (Fluorescence PCR)
Epidemiology
ʻO Isoniazid, kahi lāʻau anti-tuberculosis kī i hoʻokomo ʻia i ka makahiki 1952, ʻo ia kekahi o nā lāʻau lapaʻau maikaʻi loa no ka mālama ʻana i ka maʻi maʻi maʻi a me kahi lāʻau hoʻokahi no ka maʻi maʻi latent.
ʻO KatG ka mea nui e hoʻopili ana i ka catalase-peroxidase a me ka mutation gene katG hiki ke hoʻoikaika i ka synthesis o ka mycolic acid cell wall, e hana i ka maʻi bacteria i ka isoniazid.Hoʻopili maikaʻi ʻia ka ʻōlelo KatG me nā loli i ka INH-MIC, a ʻo ka hoʻemi ʻana o 2-fold i ka hōʻike katG e hopena i ka hoʻonui iki ʻana i 2-fold ma MIC.ʻO kekahi kumu o ka pale ʻana i ka isoniazid i ka mycobacterium tuberculosis i ka wā i hoʻokomo ʻia ai ke kumu, ka holoi ʻana a i ʻole ka hoʻololi ʻana i loko o ka InhA gene locus o mycobacterium tuberculosis.
Kanal
| ROX | inhA (-15C>T) kahua· |
| CY5 | katG (315G>C). |
| VIC (HEX) | IS6110 |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ I ka pōʻeleʻele |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | Sputum |
| CV | ≤5.0% |
| LoD | 1 × 103bacteria/mL |
| kikoo | No-cross reactivity me ka hoʻololi ʻana o nā kahua kūʻē lāʻau ʻehā (511, 516, 526 a me 531) o ka gene rpoB ma waho o ka pae ʻike o ka pahu ʻike. Nā mea hana pili: Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR Manawa maoli SLAN-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli MA-6000 ʻOiaʻiʻo ka nui o ka wela wela BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
