ʻO ka Mycobacterium Tuberculosis Rifampicin Resistance

ʻO ka wehewehe pōkole:

He kūpono kēia pahu no ka ʻike qualitative o ka homozygous mutation i loko o ka 507-533 amino acid codon māhele o ka rpoB gene i kumu kū'ē i ka Mycobacterium tuberculosis rifampicin.


Huahana Huahana

Huahana Huahana

Inoa mea kūʻai

HWTS-RT074A-Mycobacterium Tuberculosis Rifampicin Resistance Detection Kit (Fluorescence PCR)

Epidemiology

Ua hoʻohana nui ʻia ka Rifampicin i ka mālama ʻana i nā maʻi maʻi maʻi maʻi mai ka hopena o 1970s, a he hopena koʻikoʻi.ʻO ia ke koho mua e hoʻopōkole i ka chemotherapy o nā maʻi maʻi maʻi maʻi pulmonary.ʻO ke kūʻē ʻana o Rifampicin ma muli o ka hoʻololi ʻana o ka gene rpoB.ʻOiai ke puka mau nei nā lāʻau anti-tuberculosis hou, a ke hoʻomau nei ka maikaʻi o ka lāʻau lapaʻau o ka poʻe maʻi maʻi maʻi pulmonary tuberculosis, aia nō ka nele o nā lāʻau anti-tuberculosis, a ʻoi aku ka kiʻekiʻe o ke ʻano o ka hoʻohana ʻana i ka lāʻau lapaʻau ʻole.Akaka, ka Mycobacterium tuberculosis i loko o nā mea maʻi me ka pulmonary tuberculosis hiki ole loa pepehi i loko o ka manawa, ka mea hope alakai i ka likeʻole degere o ka lāʻau kū'ē i loko o ke kino o ka mea maʻi, hoʻolōʻihi i ka papa o ka maʻi, a hoʻonui i ka pilikia o ka make o ka mea maʻi.He kūpono kēia pahu no ka hōʻoia kōkua o ka Mycobacterium tuberculosis maʻi a me ka ʻike ʻana i ka rifampicin resistance gene, he mea kōkua e hoʻomaopopo i ke kūʻē ʻana i ka lāʻau o ka mycobacterium tuberculosis i hoʻopili ʻia e nā maʻi, a me ka hāʻawi ʻana i nā mea kōkua no ke alakaʻi ʻana i ka lāʻau lapaʻau.

Nā ʻāpana ʻenehana

Waihona

≤-18 ℃ I ka pōʻeleʻele

Kau-ola

9 mahina

ʻAno Hoʻohālike

Sputum

CV

≤5.0%

LoD

ʻano ʻāhiu kūʻē rifampicin: 2x103bacteria/mL

homozygous mutant: 2x103bacteria/mL

kikoo

ʻAʻohe pane keʻa o kēia pahu me ka genome kanaka, nā mycobacteria non-tuberculous ʻē aʻe, a me nā pathogens pneumonia.ʻIke ia i nā pae hoʻololi o nā genes kū'ē lāʻau ʻē aʻe o ka mycobacterium tuberculosis wild-type e like me katG 315G>C\A, InhA-15C> T, ʻaʻole hōʻike nā hopena hoʻāʻo i ke kūʻē ʻana i ka rifampicin, ʻo ia hoʻi, ʻaʻohe hopena kea.

Nā mea hana pili:

SLAN-96P Pūnaehana PCR Manawa maoli

Holo Hana

Koho 1.

Manaʻo ʻia ka hoʻoneʻe ʻana: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).

Koho 2.

Manaʻo ʻia ka mea hoʻoneʻe ʻoki: Nucleic Acid Extraction a i ʻole Purification Reagent (YDP302) na Tiangen Biotech(Beijing) Co., Ltd.


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